How does our sperm test work? Prostate Specific Antigen (PSA) is an intracellular glycoprotein synthesized only by the male prostate gland and secreted into the seminal fluid. The method uses a unique combination of labeled monoclonal conjugate (mouse) and monoclonal antibody (solid phase) to selectively identify PSA antigen in samples with high sensitivity.
As the sample passes through the absorbent paper of the semen test, the colloidal gold conjugate binds to the antigen, forming an antigen-antibody complex. This complex binds to the anti-PSA antibodies in the positive reaction window (T), the BLUESTAR® Identi-PSA® Sperm Test produces a burgundy colored band if the PSA antigen is present at a concentration greater than 4 ng/ml. If no antigen is present, no colored band appears in the window of the semen test. This protein is used in forensic medicine as a marker of choice for the detection of sexual assaults, even when committed by men who have undergone vasectomy.